This system can be used for cells and tissue sample light field, dark filed, phase error, Simple polarization and epifluorescence observation. And, can observe and research fine structure of cells without influence on living cells, which can be used to research small molecule fluorescent probe and fluorescent protein, like Fluorescent probe subcellular localization, cell apoptosis detection, immunofluorescent staining (Immunocytochemistry，Immunohistochemistry), ISH FRET, HCS and photobleaching, photoactivation . The software of this system is NIS-Elements, which support for all kinds of image shoot, analysis mode.