In-Vivo multispectral imaging system can detect special biological behaviour non-invasively in vivo, like cell proliferation or migration, gene expression, the movement and metabolism of small molecular, ect. The signal of this instrument contains fluorescence signal, luminous signal and radioactive isotope signal. The kinds of sample include living animal, cell, clone, protein, gel, albumen glue, ect. The system can provide more selection on research method and direction.